Prof. Hu's Research Group

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  Previous Research Project Highlights (2004~2012)


        Silk-tropoelastin composite protein materials are produced as an example to demonstrate the cell-material interactions, related to mechanics (bone cells), surface morphology (muscle cells) and surface charge (neuron cells).

Representative Publications:

Xiao Hu, Min D. Tang-Schomer, Wenwen Huang, Guokui Qin, Xiao-Xia Xia, Anthony S. Weiss, David L. Kaplan. Charge-Tunable Autoclaved Silk-Tropoelastin Protein Alloys That Control Neuron Cell Responses.  Advanced Functional Materials (2013), in press (DOI: 10.1002/adfm.201202685).

(Review) Xiao Hu, Peggy Cebe, Anthony S. Weiss, Fiorenzo Omenetto, David L. Kaplan. Protein-Based Composite Materials. Materials Today, 15(5), 208-215, (2012).

Xiao Hu*, Sang-Hyug Park*, Eun Seok Gil, Xiao-Xia Xia, Anthony S. Weiss, David L. Kaplan. The influence of elasticity and surface roughness on myogenic and osteogenic-differentiation of cells on silk-elastin biomaterials. Biomaterials, 32(34), 8979-89, (2011). (*Equal contribution)

Xiao Hu, Xiuli Wang, Jelena Rnjak, Anthony S. Weiss, David L. Kaplan. Biomaterials derived from silk-tropoelastin protein systems. Biomaterials, 31(32), 8121-8131, (2010).



           The control of protein self-assembly process is critical, as this provides insight into molecular level impact on biological functions and physical properties of materials. For example, through revealing self-assembly mechanism of nature resilin proteins (a polymeric protein existed in flight and jumping organs of insects), we can recombine new resilin-like peptides and mimic their micelle assembly process by hydrophilic-hydrophobic interactions and beta-turn structural transitions. These resilin protein materials can function as ¡°super elastic bio-rubbers¡± with more than 90% resilience, and can extend and retract thousands of times with rapid energy transformations.        

Representative Publications:

Guokui Qin*/ Xiao Hu*, Peggy Cebe, David Kaplan. Mechanism of resilin elasticity. Nature Communications, (2012). (*Equal contribution)

Guokui Qin*, Amit Rivkin*, Shaul Lapidot, Xiao Hu, Shira B. Arinos, Or Dgany, Oded Shoseyov, David L. Kaplan. Recombinant Exon-Encoded Resilins for Elastomeric Biomaterials. Biomaterials, 32(35), 9231-9243, (2011). (*Equal contribution)

Guokui Qin*, Shaul Lapidot*, Keiji Numata, Xiao Hu, Sigal Meirovitch, Mara Dekel, Itai Podoler, Oded Shoseyov, David Kaplan. Expression, cross-linking and characterization of recombinant chitin binding resilin. Biomacromolecules, 10, 3227-3234, (2009). (*Equal contribution)



o    Biophysics of Bombyx Mori Silk Materials (with Prof. Peggy Cebe, Prof. David Kaplan, Prof. Christoph Schick, Prof. M. Pyda etc.)

        We have developed variable methods to dynamically characterize structures and properties of Bombyx Mori silk protein materials, including Temperature modulated Differential Scanning Calorimetry (TMDSC), Time-resolved Fourier Transform Infrared Microscopy (RT-FTIR), Fourier-transferred self-deconvolution of protein infrared spectrum (FSD), synchrotron real-time wide-angle/small-angle X-ray (WAXS/SAXS), Circular Dichroism (CD), Dynamic Mechanical Analysis (DMA), Dielectric Analysis (DEA), Nuclear Magnetic Resonance (NMR), Fluorescence Spectrometry, and real-time Atomic Force or Scanning Electron Microscopy (AFM /SEM) etc.

Representative Publications:

Peggy Cebe, Xiao Hu, David Kaplan, Evgeny Zhuravlev, Andreas Wurm, Daniella Arbeiter, Christoph Schick. Beating the Heat - Fast Scanning Melts Beta Sheet Crystals. (Nature) Scientific Reports, 3, 1130, (2013).

Book Chapter: Xiao Hu and David L. Kaplan. Comprehensive Biomaterials: Vol. 2, 2.212 Silk Biomaterials, Editors-Paul Ducheyne, Kevin E Healy, Dietmar E Hutmacher etc.- Elsevier Ltd.,  Pages 207-219, (2011). (Print ISBN: 9780080553023; Electronic ISBN: 9780080552941).

Wenwen Huang, Sreevidhya Krishnaji, Xiao Hu, David Kaplan, Peggy Cebe. Heat Capacity of Spider Silk-like Block Copolymers. Macromolecules, 2011, 44(13), 5295-5305, (2011).

Xiao Hu, Karen Shmelev, Lin Sun, Eun-Seok Gil, Sang-Hyug Park, Peggy Cebe, David L. Kaplan. Regulation of Silk Material Structure by Temperature-Controlled Water Vapor Annealing. Biomacromolecules, 12(5), 1686-1696, (2011).

Xiao Hu, Qiang Lu, David Kaplan, Peggy Cebe. Microphase Separation Controlled Beta Sheet Crystallization Kinetics in Fibrous Proteins. Macromolecules, 42 (6), 2079-2087, (2009).

Lei Yu, Xiao Hu, David Kaplan, Peggy Cebe. Dielectric Relaxation Spectroscopy of Hydrated and Dehydrated Silk Fibroin Cast from Aqueous Solution. Biomacromolecules, 11(10), 2766-2775, (2010).

Xiao Hu, David Kaplan, Peggy Cebe. Dynamic Protein-Water Relationships during Beta Sheet Formation.  Macromolecules, 41(11), 3939-3948, (2008).

M. Pyda, Xiao Hu, Peggy Cebe. Heat Capacity of Silk Fibroin Based on the Vibration-Motion of Poly(amino acid)s in the Absence and in the Presence of Water. Macromolecules, 41(13), 4786-4793, (2008).

Xiao Hu, David Kaplan, Peggy Cebe. Determining Beta Sheet Crystallinity in Fibrous Proteins by Thermal Analysis and Infrared Spectroscopy. Macromolecules, 39, 6161-6170 (2006).



o    Biomaterials for Tissue Regeneration and Drug Delivery (with Prof. David Kaplan, Prof. Qiang Lu, Dr. Tara D. Sutherland, Prof. Xungai Wang, Prof. Thomas Scheibel, Prof. Joyce Wong, Prof. Qiaobing Xu, Prof. Antonella Motta etc.)


Representative Publications:

Jeney Zhang*, Eleanor Pritchard*, Xiao Hu, Thomas Valentin, Bruce Panilaitis, Fiorenzo G. Omenetto, David L. Kaplan. Stabilization of vaccines and antibiotics in silk and eliminating the cold chain. PNAS (Proceedings of the National Academy of Sciences of the United States of America), 109(30), 11981-11986, (2012). (*Equal contribution)

Rangam Rajkhowa, Xiao Hu, Takuya Tsuzuki, David L. Kaplan, Xungai Wang. Structure and Biodegradation Mechanism of Milled Bombyx mori Silk Particles. Biomacromolecules, 13(8), 2503-2512, (2012).

Danyu Yao, Sen Dong, Qiang Lu, Xiao Hu, David L Kaplan, Bingbo Zhang, Hesun Zhu. Salt-Leached Silk Scaffolds with Tunable Mechanical Properties. Biomacromolecules, 13(11), 3723-3729, (2012).

A.L. Oliveira, L. Sun, H.J. Kim, Xiao Hu, W. Rice, J. Kluge, R. L. Reis and D. L. Kaplan  Aligned Silk-Based 3D Architectures for Contact Guidance in Tissue Engineering. Acta Biomaterialia, 8(4), 1530-1542, (2012).

F. Philipp Seib*, Manfred F. Maitz*, Xiao Hu, Carsten Werner, David L. Kaplan Impact of processing parameters on the haemocompatibility of Bombyx mori silk films. Biomaterials, 33, 1017-1023, (2012). (*Equal contribution)

Shuo Sun, Ming Wang, Sarah Knupp, Yadira Soto-Feliciano, Xiao Hu, David Kaplan, Robert Langer, Daniel Anderson, Qiaobing Xu.  Combinatorial Library of Lipidoids for In Vitro DNA Delivery. Bioconjugate Chemistry, 23(1), 135-140, (2012).

Cencen Zhang, Dawei Song, Qiang Lu, Xiao Hu, David L. Kaplan, Hesun Zhu. Flexibility Regeneration of Silk Fibroin in Vitro. Biomacromolecules, 13(7), 2148-2153, (2012).

Tara D. Sutherland, Jeffrey S. Church, Xiao Hu, Mickey G. Huson, David L. Kaplan, Sarah Weisman. Single honeybee silk protein mimics properties of multi-protein silk. PLoS ONE, 6(2), e16489, (2011).

Xiao-Xia Xia, Qiaobing Xu, Xiao Hu, Guokui Qin, and David L. Kaplan. Tunable Self-Assembly of Genetically Engineered Silk-Elastin-Like Protein Polymers. Biomacromolecules, 12(11), 3844-3850, (2011).

Michelle Kinahan, Emmanouela Filippidi, Sarah Koster, Xiao Hu, Heather M. Evans, Thomas Pfohl, David L. Kaplan, Joyce Wong. Tunable Silk: Using Microfluidics to Fabricate Silk Fibers with Controllable Properties. Biomacromolecules, 12(5), 1504-1511, (2011).

Corinne R. Wittmer, Xiao Hu, Pierre-Chanel Gauthier, Sarah Weisman, David L. Kaplan, Tara D. Sutherland. Production, structure and in vitro degradation of electrospun honeybee silk nanofibers. Acta Biomaterialia, 7(10), 3789-3795, (2011).

Andreas Lammel, Xiao Hu, Sang-Hyug Park, David L. Kaplan, Thomas Scheibel. Controlling silk fibroin particle features for drug delivery. Biomaterials, 31, 4583-4591, (2010).

Xiao Hu, Qiang Lu, Lin Sun, Peggy Cebe, Xiaoqin Wang, Xiaohui Zhang, David Kaplan. Biomaterials from Ultrasonication-Induced Silk Fibroin-Hyaluronic Acid Hydrogels. Biomacromolecules, 11, 3178-3188, (2010).

Qiang Lu, Xiao Hu, Xiaoqin Wang, Jonathan Klugea, Shenzhou Lu, Peggy Cebe, David L. Kaplan. Water-Insoluble Silk Films with Silk I Structure. Acta Biomaterialia, 6, 1380-1387, (2009).

Xiaoqin Wang, Tuna Yucel, Qiang Lu, Xiao Hu, David L. Kaplan. Silk Nanospheres and Microspheres from Silk/PVA Blend Films. Biomaterials, 31, 1025-1035, (2009).

Shenzhou Lu, Xiaoqin Wang, Qiang Lu, Xiao Hu, Neha Uppal, Fiorenzo Omenetto, David Kaplan. Stabilization of Enzymes in Silk Films. Biomacromolecules, 10(5), 1032¨C1042, (2009).

Antonella Motta, Devid Maniglio, Claudio Migliaresi, Hyeon-Joo Kim, Xianyan Wang, Xiao Hu, David Kaplan. Silk Fibroin Processing and Thrombogenic Responses. J. Biomaterials Science, Polymer Edition, 20 (13), 1875-1897, (2009).

Xiaoqin Wang, Esther Wenk, Xiao Hu, Guillermo R. Castro, Lorenz Meinel, Xianyan Wang, Chunmei Li, Hans Merkle, David Kaplan. Silk coatings on PLGA and alginate microspheres for protein delivery. Biomaterials, 28,  4161-4169, (2007).

Xianyan Wang, Xiao Hu, Andrea Daley, Olena Rabotyagova, Peggy Cebe, and David Kaplan. Nanolayer biomaterial coatings of silk fibroin for controlled release. Journal of Controlled Release, 121, 190-199, (2007).